Radioimmunoassay of bleomycins.

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Radioimmunoassay of bleomycins.

Rabbit antisera highly specific to the bleomycinic acid moiety of bleomycins were obtained by immunizing with a conjugate of copper-complex of bleomycin A5 and bovine serum albumin. These antisera not only reacted with bleomycin A5 but also with other bleomycins such as bleomycin A2, bleomycin B2 and peplomycin. The antisera showed little cross-reactivity with deamido-, depyruvamido- and decarb...

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Biosyntheses of new bleomycins.

Bleomycins, a group of glycopeptide antibiotics differ from one another in their terminal amine moieties. 14C-Methyl-labeled 3-aminopropyl-dimethylsulfonium was confirmed to be incorporated into the amine moiety of bleomycin A2. Addition of amines of bleomycin A2, A2'-a, A2'-b, A2'-c, A5, B2 and B4 to fermentation media increased the rate of production of the bleomycin which contained the added...

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Positive interference in Radioimmunoassay determination of serum total T3

Introduction: Determination of thyrotropin, total and free thyroxine and triiodothyronine are widely used as diagnostic methods for thyroid function evaluation. There have been numerous reports of interferences in thyroid hormone immunoassays. Herein, a prominent positive interference is reported for a radioimmunoassay kit of total triiodothyronine. Materials and Methods: In this study, a total...

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Natural and artificial bleomycins: chemistry and antitumor activities.

The bleomycins are a group of antibiotics produced by Streptomyces verticillus and their therapeutic effect against the squamous cell carcinoma has been proved by clinical studies. In our laboratory, eight products, viz. bleomycin A1, demethyl-A2, A-a, A-b, B2, A5 and B4 have been isolated in the pure state. Structures of the various bleomycins have been elucidated and it has been observed that...

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ژورنال

عنوان ژورنال: The Journal of Antibiotics

سال: 1985

ISSN: 0021-8820,1881-1469

DOI: 10.7164/antibiotics.38.1251